Journal: BMB Reports

Article Title: Regorafenib prevents the development of emphysema in a murine elastase model

doi: 10.5483/BMBRep.2023-0072

Figure Lengend Snippet: Effect of regorafenib on PPE-induced in inflammatory mediators in BAL fluid. (A) Representative membrane images of the antibody array. (B) Quantification of relative optical density for IL-1β, IL-6, CXCL1/KC, and TIMP-1 in the array membranes. (C-F) Measurement of IL-1β (C), IL-6 (D), CXCL1/KC (E), and TIMP-1 (F) levels in BAL fluid by ELISA. Error bars indicate the mean ± SEM. N = 8 per group, *P < 0.05, **P < 0.01, ****P < 0.0001, t -test.

Article Snippet: Anti-IL-6 antibody (ab208113) from abcam (Cambridge, UK), CXCL1 antibody (12335-1-AP) from Proteintech (Rosemont, IL, USA), and TIMP-1 antibody (#AF980) from R&D Systems were purchased respectively.

Techniques: Membrane, Ab Array, Enzyme-linked Immunosorbent Assay

Journal: BMB Reports

Article Title: Regorafenib prevents the development of emphysema in a murine elastase model

doi: 10.5483/BMBRep.2023-0072

Figure Lengend Snippet: Effect of regorafenib on PPE-induced expression of inflammatory mediators in lung parenchyme. (A) Experimental scheme. Mice were sensitized by treatment with regorafenib (5 mg/kg) for one day in the only PPE + Reg group. After PPE treatment, regorafenib was administered orally to mice for six days. On the seventh day, lung tissues were removed from mice, prepared in paraffin sections and stained with antibodies for inflammatory mediators. (B) Representative images of stained paraffin sections. Red arrows indicate the macrophages in inserted boxes. Scale bars indicate 50 μm. (C-E) Quantification of . IL-6 (C), CXCL1/KC (D), and TIMP-1 (E). Error bars indicate the mean ± SEM. N = 5 per group, *P < 0.05, **P < 0.01, t -test.

Article Snippet: Anti-IL-6 antibody (ab208113) from abcam (Cambridge, UK), CXCL1 antibody (12335-1-AP) from Proteintech (Rosemont, IL, USA), and TIMP-1 antibody (#AF980) from R&D Systems were purchased respectively.

Techniques: Expressing, Staining

Journal: Frontiers in Cell and Developmental Biology

Article Title: Acetylcholine in the gingival epithelium drives the pathogenesis of periodontitis

doi: 10.3389/fcell.2025.1701252

Figure Lengend Snippet: Acetylcholine orchestrates a multi-dimensional defense program in epithelial cells. (A) Network of GSEA-GO enrichment analysis for Pg vs. NC DEGs. Each node represents a GO term, with circle size and color indicating the pathway size and the NES score, respectively. Node-sharing clusters represent functionally related term groups. (B) Transcript counts of CXCL and CCL family genes in HOKs RNA-seq data. Only genes with non-zero counts across all 12 samples are displayed, with orange bars indicating the mean. (C) Volcano plot of DEGs in Pg vs. NC comparison. Colored dots represent significantly differential expression (|log2 fold change| > 1, adjusted p -value <0.05). (D) Volcano plot of PD vs. HC DEGs in epithelial cells from single-cell data. Colored dots represent significantly differential expression (|log2 fold change| > 1, adjusted p -value <0.05). (E) Enzyme-linked immunosorbent assay (ELISA) results of HOK supernatants for CXCL1 and CXCL8. *, p < 0.05 and **, p < 0.01. (F) Enrichment analysis of DEGs from the Pg + Ach vs. Pg groups using the fgsea function, showing four significantly enriched functional categories [adjusted p < 0.05, normalized enrichment score (NES) > 1.5].

Article Snippet: The levels of CXCL1 and CXCL8 were then measured using an enzyme-linked immunosorbent assay (ELISA) kit (Proteintech, KE00133 and KE00453), according to the manufacturer’s instructions.

Techniques: RNA Sequencing, Comparison, Quantitative Proteomics, Enzyme-linked Immunosorbent Assay, Functional Assay

Journal: Cell Death & Disease

Article Title: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

doi: 10.1038/s41419-025-07841-4

Figure Lengend Snippet: A Venn diagram of the intersection of differentially expressed genes from three HPI gastritis datasets. B Heatmap of the expression of the 7 intersecting differentially expressed genes in the GSE5081 dataset (CXCL1-Low group: n = 4; CXCL1-High group: n = 4). C Heatmap of the expression of the 7 intersecting genes in the GSE60427 dataset (CXCL1-Low group: n = 8; CXCL1-High group: n = 8). D Heatmap of the expression of the 7 intersecting genes in the GSE60662 dataset (CXCL1-Low group: n = 4; CXCL1-High group: n = 4). E GO functional analysis of the 7 intersecting differentially expressed genes in terms of biological process (BP), cellular component (CC), and molecular function (MF). F KEGG pathway enrichment analysis of the 7 intersecting differentially expressed genes. G Heatmap of the expression of 3 differentially expressed m6A modification-related genes in the GSE5081 dataset (Control group: n = 8; Gastritis group: n = 8). H Heatmap of the expression of 3 differentially expressed m6A modification-related genes in the GSE60427 dataset (Control group: n = 8; Gastritis group: n = 16). I Heatmap of the expression of 3 differentially expressed m6A modification-related genes in the GSE60662 dataset (Control group: n = 4; Gastritis group: n = 8). J Venn diagram of the intersection of differential genes related to m6A modification in three HPI gastritis datasets. K Correlation analysis of METTL3 and CXCL1 in the GSE5081 dataset. L Correlation analysis of METTL3 and CXCL1 in the GSE60427 dataset. M Correlation analysis of METTL3 and CXCL1 in the GSE60662 dataset. Values are expressed as mean ± standard deviation. * P < 0.05, ** P < 0.01, *** P < 0.001 compared to Control.

Article Snippet: All the antibodies mentioned above, except CXCL1 (purchased from Protein Tech), were obtained from Abcam.

Techniques: Expressing, Functional Assay, Modification, Control, Standard Deviation

Journal: Cell Death & Disease

Article Title: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

doi: 10.1038/s41419-025-07841-4

Figure Lengend Snippet: A Western blot analysis of CXCL1 and METTL3 protein levels in GES-1 cells after co-culture with HP at different MOIs (0, 20, 50, 100, and 200) and for different durations (0, 3, 6, 12, and 24 h). B RT-qPCR analysis of CXCL1 and METTL3 mRNA levels in GES-1 cells after co-culture with HP at different MOIs (0, 20, 50, 100, and 200) and for different durations (0, 3, 6, 12, and 24 h). C RT-qPCR and Western blot screening of three si-METTL3 sequences. D MeRIP-qPCR experiment detecting m6A methylation modifications of CXCL1 mRNA in GES-1 cells. E RIP-qPCR experiment detecting the target relationship between CXCL1 mRNA and METTL3 in GES-1 cells. F Dual-luciferase reporter assay detecting the target relationship between CXCL1 and METTL3 in GES-1 cells. G Western blot analysis of CXCL1 and METTL3 protein expression levels in HP-infected GES-1 cells. H RT-qPCR analysis of CXCL1 and METTL3 mRNA levels in HP-infected GES-1 cells. I Immunofluorescence staining detecting the expression levels of CXCL1 and METTL3 in HP-infected GES-1 cells. J MeRIP-qPCR experiment detecting m6A methylation modifications of CXCL1 mRNA in GES-1 cells. K RIP-qPCR experiment detecting the target relationship between CXCL1 mRNA and BHLHE41 in GES-1 cells. L Dual-luciferase reporter assay detecting the target relationship between CXCL1 and BHLHE41 in GES-1 cells. M Western blot analysis of CXCL1 and BHLHE41 protein expression levels in HP-infected GES-1 cells. N RT-qPCR analysis of CXCL1 and BHLHE41 mRNA levels in HP-infected GES-1 cells. O Immunofluorescence staining detecting the expression levels of CXCL1 and BHLHE41 in HP-infected GES-1 cells. Scale bar: 25 μm. Values are presented as the mean ± standard deviation, and all cell experiments were repeated three times. ***indicates P < 0.001.

Article Snippet: All the antibodies mentioned above, except CXCL1 (purchased from Protein Tech), were obtained from Abcam.

Techniques: Western Blot, Co-Culture Assay, Quantitative RT-PCR, Methylation, Luciferase, Reporter Assay, Expressing, Infection, Immunofluorescence, Staining, Standard Deviation

Journal: Cell Death & Disease

Article Title: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

doi: 10.1038/s41419-025-07841-4

Figure Lengend Snippet: A RT-qPCR screening of three si-CXCL1 sequences. B Western blot analysis of p-IκBα, IκBα, p-p65, and p65 protein expression levels in HPI-treated GES-1 cells. The bar charts represent the ratios of p-IκBα/IκBα and p-p65/p65 protein levels. C Immunofluorescence staining of CXCL1 and NF-κB expression levels in HPI-treated GES-1 cells, scale bar: 25 μm. Values are presented as the mean ± standard deviation, and all cell experiments were repeated three times. *** indicates P < 0.001.

Article Snippet: All the antibodies mentioned above, except CXCL1 (purchased from Protein Tech), were obtained from Abcam.

Techniques: Quantitative RT-PCR, Western Blot, Expressing, Immunofluorescence, Staining, Standard Deviation

Journal: Cell Death & Disease

Article Title: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

doi: 10.1038/s41419-025-07841-4

Figure Lengend Snippet: A Construction of METTL3 −/− and CXCL1 −/− mice using CRISPR-Pro gene knockout technology, along with RT-qPCR and sequencing results. B Rapid urease test to detect gastric mucosal infection in mice. C H&E staining to observe the morphology and structure of gastric mucosal cells, scale bar: 50 μm. D TUNEL assay to detect apoptosis in gastric mucosal cells, scale bar: 25 μm. E Western blot analysis of cleaved caspase-3, cleaved caspase-9, and cleaved PARP levels. F RT-qPCR analysis of CXCL1, IL-6, IL-8, and TNF-α mRNA levels in the gastric mucosa of mice. G ELISA analysis of CXCL1, IL-6, and TNF-α levels in the gastric mucosa of mice. H Immunofluorescence detection of GIF expression in the gastric mucosa, scale bar: 25 μm. I Immunofluorescence detection of Ki67 expression in the gastric mucosa, scale bar: 25 μm. J Immunofluorescence detection of MPO expression in the gastric mucosa, scale bar: 25 μm. Each group contains 6 mice. Values are presented as the mean ± standard deviation, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

Article Snippet: All the antibodies mentioned above, except CXCL1 (purchased from Protein Tech), were obtained from Abcam.

Techniques: CRISPR, Gene Knockout, Quantitative RT-PCR, Sequencing, Infection, Staining, TUNEL Assay, Western Blot, Enzyme-linked Immunosorbent Assay, Immunofluorescence, Expressing, Standard Deviation

Journal: Cell Death & Disease

Article Title: Unraveling the Role of METTL3 in Helicobacter pylori-induced gastritis via m6A-CXCL1/NF-κB modulation

doi: 10.1038/s41419-025-07841-4

Figure Lengend Snippet: A Western blot analysis of the protein levels of METTL3, CXCL1, p-IκBα, IκBα, p-p65, and p65 in gastric mucosa of mice. B METTL3, CXCL1 and ratios of p-IκBα/IκBα, p-p65/p65 protein levels. C Immunohistochemical staining to detect the expression of CXCL1 in gastric mucosa of mice, scale bar: 50 μm. D Immunofluorescence staining to assess the expression levels of p65 in gastric mucosa of mice, with the right images showing enlarged views of the left images, scale bar: 50 /25 μm. E Immunofluorescence staining to assess the expression levels of p-p65 in gastric mucosa of mice, with the right images showing enlarged views of the left images, scale bar: 50 /25 μm. Each group consisted of 6 mice, values are presented as mean ± standard deviation, * indicates P < 0.05, ** indicates P < 0.01, *** indicates P < 0.001.

Article Snippet: All the antibodies mentioned above, except CXCL1 (purchased from Protein Tech), were obtained from Abcam.

Techniques: Western Blot, Immunohistochemical staining, Staining, Expressing, Immunofluorescence, Standard Deviation